Ocular Microbiology and Immunology Group
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2018 OMIG Abstract

Evaluating the Antimicrobial Effect of Amniotic Membrane Grafts

Michele Lee, MD^1,2, Yijie Lin, MD MBA^1,2, Victoria Cavallos, MT¹ Jeremy Keenan, MD MPH^1,2,
Gerami Seitzman, MD^1,2
1 Frances I. Proctor Foundation for Research in Ophthalmology, San Francisco, CA,
² Department of Ophthalmology, University of California San Francisco, San Francisco, CA

Methods: In this in vitro study, McFarland dilutions of Staphylococcus aureus, Streptococcus pneumoniae, and Pseudomonas aeruginosa were plated uniformly on blood agar plates. Each plate was divided into four quadrants: 1) AmbioDisk (IOP Ophthalmics/Katena, Costa Mesa, CA), 2) AmbioDisk with overlying contact lens, 3) sterile Whatman filter paper disk and 4) sterile disk with a micro-volume drop of moxifloxacin 0.5%. Plates were incubated at 37? Celsius. Zones of inhibition were read for each group at 20 hours. This was performed in triplicate for each organism. In addition, acanthamoeba was incubated for 24 hours with, chlorhexidine, solubilized amniotic membrane, and control saline. After serial dilutions, acanthamoeba was plated on a non-nutrient plate with E.coli overlay and incubated for 48 hours. Presence of trophozoites was assessed using an inverted microscope.

Results: There was no inhibition of growth against Pseudomonas aeruginosa and Streptococcus pneumoniae with either the AmbioDisk, AmbioDisk with contact lens, or sterile disk. There was minimal growth inhibition of Staphylococcus aureus using AmbioDisk (average zone of inhibition of 12.7mm), AmbioDisk with contact lens (14.3mm), but this is well below the CLSI performance standards for antimicrobial sensitivity. Using the Monte Carlo permutation test, we found that there was not a statistically significant difference when comparing the zone of inhibition of the control (sterile disk) with AmbioDisk (p=0.25) or AmbioDisk with contact lens (p=0.25). Amniotic membrane did not inhibit growth of acanthamoba trophozoites.

Conclusion: In vitro, amniotic membrane does not show convincing antimicrobial activity. Based on these results, we do not advocate the use of amniotic membrane grafts as antimicrobial agents.

Disclosure: N; Katena donated AmbioDisk for the project.

2018 Agenda and Abstracts | < Previous Next >